Plasmid Profile Analysis of Salmonella Enterica Serotype Enteritidis

Plasmid profile analysis (PP) is a method of determining a number and size of plasmids in bacterial isolates. When using plasmid profile analysis in determining epidemiological strain for evaluation of this method, it is necessary to compare epidemiological strains with non-epidemiological ones isolated in the same period of time. Plasmid profile determination is performed by lysis of bacterial cell, chromosomal denaturation, sedimentation of cell fragments by centrifugation, and precipitation of DNA with ethanol. DNA is separated by gel electrophoresis based on its molecule mass. Plasmids could be also analyzed by restriction enzymes, as well as by direct techniques, such as hybridization and electronomicroscopically. Using of plasmid profile analysis enables resolving numerous outbreaks caused by Enterobacteriaceae, especially Salmonella. This method could be performed alone for Salmonella enterica serotype Enteritidis, but also as a complementary method to phage typing (phagotypization), and other molecular genetic based techniques. Acta Medica Medianae 2008;47(2):54-57.